WARNING Failure to keep the mat clear of plates, tubes or any other materials may result in damage to the BD CSampler. the BD Accuri C6 cytometer, saving time. Browse For Folder Dialog Box, Page 27: Figure 5-3. The BD Accuri C6 Plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the single-cell level. Our neuroimmunology lab currently has a BD Accuri C6 that has broken about every year and a half since it’s purchase (about 5 yrs old). endobj Use another characteristic of the particle, such as fluorescence, to �&�Yl�H�$��9It��$�%�|X��̒��L���B��D��kt��/��#_� )a!�X܌��N�Xb��CF���D'�)A�K�ȃq��j���T����L�QO��< ��a�� (In this example, the file is FCS Express Import Demo.c6.) Service for BD Accuri C6 systems and their related options will continue until April 15, 2021. Frequency Task Supplies and parts After every experiment Run filtered DI water at the Fast rate for 3 to 5 minutes to prevent clogging. Satchmo stanford shared facs facility (9 pages), Manual will be automatically added to "My Manuals", Laboratory Equipment BD CSampler Installation Manual, Laboratory Equipment BD LSR II User Manual, Laboratory Equipment BD FACSJAZZ User Manual, Laboratory Equipment BD FACSCanto User Manual, Laboratory Equipment BD FACSCanto Reference Manual, Laboratory Equipment BD FACSAria II Quick Reference Manual, Laboratory Equipment BD FACSort User Manual, Laboratory Equipment BD FACScan Instructions, Laboratory Equipment BD 644787 User Manual, Page 5: Figure 1-1. The microprocessor-controlled peristaltic pump system allows the sample volume pulled per run to be determined. Flow cytometry should be easy. Depress#the#Power#button#on#the#BD#Accuri#C6#instrumentface#panel#for#1#second.#The# instrumentwill#begin#a5#minute#auto#startcycle.#If#the#BD#Accuri#software#displays#the# message#Extra!Start,up!time!needed!due!to!cleaning!or!improper!shutdown! User's Guides and Manuals. To download BD ACCURI C6 MANUAL… BD Accuri™ C6 Plus Filter Guide Product Information Sheets. Scheduling. 4 0 obj BD Accuri C6 Software User Guide - Manual vi 7820095-01 Rev-0 Table B-1. BD CSampler Software User Guide iv 7820099-01 Rev-2 TABLE OF FIGURES Figure 1-1. By providing this information you also agree to receive BD Accuri News, our monthly electronic newsletter. Software options … Only operate the Accuri C6 Flow Cytometer with an original copyrighted version of software authorized by Accuri. %PDF-1.5 Start the Accuri CSampler software. Operate the instrument as described in this Instruction Manual. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. 2 0 obj Hold the BD CSampler module with both hands and locate the tab in the slot on the back of the BD Accuri C6 (Figure 5). Plug in the power cord and turn on the C6. 11. DI water, Cat. BD Accuri C6 Flow Cytometer Instrument Manual 7820018-01 Rev-2 1 The BD Accuri™ C6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. Of the BD Accuri C6 Flow Cytometer Instrument Manual Research Use Only. BD ACCURI C6 MANUAL might not make exciting reading, but BD ACCURI C6 MANUAL comes complete with valuable specification, instructions, information and warnings. Install BD CSampler software. stream To perform compensation, 1. A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file. BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express™ software and FlowJo™ software. BD Accuri C6 Plus software features an intuitive user interface. BD Accuri™ C6 Flow Cytometer Optical Filter Guide Recommended optical choices for common fluorophores Dyes Laser Standard Filters Detector Optional Filters2 FITC, BD Horizon Brilliant™ Blue 515, Alexa Fluor® 488, CFSE 488 nm 533/30 FL1 — GFP3 488 nm 533/30 FL1 510/15 YFP3 488 nm 533/30 FL1 540/20 PE 488 nm 585/40 FL2 — OFP 488 nm 585/40 FL2 565/20 PI 488 nm 585/40, 670 LP FL2, FL3 … Print version: Text Size: Related Files » C6 manual » BC aquatic uses ... Accuri C6 consumables Consumables (fluids): (MSDS linked at the right -->) Cleaning solution, KR-225 (citric acid). 12. Install, update, and run anti-virus protection software on a regular basis. Within 5 minutes of completing the water run, place a calibration sample on the SIP. If you can simplify cell analysis ITC Data Analysis in versions 8. If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for Multiplex assays page, or contact our Technical Support team at technical@abcam.com. It Also Includes Common Troubleshooting Help And Service Information. BD Accuri® - Guide to the FCS Express™ BD Accuri CFlow® Importer Instruction Manual Subject: The FCS Express BD Accuri CFlow Importer, available in FCS Express version 4, allows you to automatically recreate any analysis scheme from BD Accuri CFlow® or BD Accuri CFlow Plus software in FCS Express. Windows Security Window. BD Accuri C6 Flow Cytometer Instrument Manual 7820018-01 Rev-2 1 The BD Accuri™ C6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. BD ACCURI C6 MANUAL might not make exciting reading, but BD ACCURI C6 MANUAL comes complete with valuable specification, instructions, information and warnings. Figure 6 shows how the FCS file data will look when initially opened in FlowJo. Post-acquisition compensation can be performed using Accuri C6 software Page 2/13. BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express™ software and FlowJo™ software. x��XKo�H�#��� M��^EH �D�H���=x�!삙�1Q��V� ��m8ەz~]U]ݤ? BD Accuri C6 Plus software features an intuitive user interface. BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. Sliding Luer Lock Fittings Off Of Pump Head, Page 25: Troubleshooting And Technical Support, Page 26: Figure 5-1. BD Accuri C6: BMSB J538. Canada United States Get Free Accuri C6 User Guide or third-party applications. Holes Figure 4 Figure 5 Figure 6 5. Place the mat in position (Figure 10). Service for BD Accuri C6 systems and their related options will continue until April 15, 2021. 新一代的Accuri使流式細胞術更加指日可待。 Taiwan North America. Systems has broken about every year and for core immunophenotyping studies and services from each plot. To install BD Accuri C6 Software, run BDAccuriC6SoftwareInstaller.exe. 9. Technology Has Developed, And Reading Bd Accuri Analysis Manual Printable 2019 Books … • For Information On Installing And Using The BD CSampler ™, See The BD Accuri CSampler … The BD Accuri™ C6 is a dual laser flow cytometer capable of detecting four distinct fluorochromes simultaneously. BD Accuri™ C6 Flow Cytometer Instruction Manual, serial number 2400 and higher; BD Accuri™ C6 Software User Guide; BD CSampler™ Accessory Installation Guide; BD CSampler™ Software User Guide; Display of BD Accuri™ C6 Software-Generated FCS 3.0 Files Using FlowJo™ Version 8.8.7 or 9.4.11 for the Mac® 4 This filter transmits red laser light, which may damage the detector. Waste Line Quick Disconnect, Page 22: Figure 4-3. <>/XObject<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 720 540] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> In-Line Sheath Filter, Page 13: Figure 3-5. As understood, achievement does not recommend that you have fantastic points. Select Instrument > Calibrate Fluidics. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. Turn on the computer and log in with your UCF info. NOTE: The BD Accuri CFlow Importer reads the FCS data directly from the BD Accuri CFlow file, so you do not have to export individual files. Default cytometer configuration for the Accuri C6 analyzer: For more information click the following links: C6 cytometer user manual. If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for Multiplex assays page, or contact our Technical Support team at technical@abcam.com. Core Size/Flow Rate Combinations.....79 Figure C-1. The Accuri C6 Flow Cytometer® simplifies cell analysis by performing absolute cell counts, eliminating the need for counting beads, or dual-platform hemacytometer counts. endobj Download: Bd Accuri Analysis Manual Printable 2019 Online Reading At BRAZILFILMFESTIVAL.INFO Free Download Books Bd Accuri Analysis Manual Printable 2019 We All Know That Reading Bd Accuri Analysis Manual Printable 2019 Is Beneficial, Because We Can Easily Get A Lot Of Information From Your Resources. Download Ebook Bd Accuri C6 Bd Accuri C6 Yeah, reviewing a books bd accuri c6 could amass your near contacts listings. The Bd Accuri C6 Flow Cytometer System, Page 6: Bd Accuri C6 Flow Cytometer Specifications, Page 9: Figure 3-1. <> The BD CSampler will auto- matically align itself and then remain in the home position (Figure 8 - 9). REQUEST A DEMO ON A BD ACCURI C6 SYSTEM. Automated Preventive Maintenance Notification Window, Page 21: Figure 4-2. • For Information On Using BD Accuri C6 Software, See The BD Accuri C6 Software User Guide. BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. A Guide to Absolute Counting Using the BD Accuri® C6 Flow Cytometer BD Biosciences August 2011 Page 3 5. The BD Accuri C6 Flow Cytometer System . 7820095-01 Rev-0 51. 3 VALIDATING THE PERFORMANCE OF THE BD ACCURI C6 FLOW CYTOMETER .....11 3.1 Running Validation Beads (Daily Start-Up) .....11 3.2 Saving Validation Bead Data .....15 3.3 Analyzing and Recording Validation Bead Data.....15 3.4 Monitoring Validation Bead Data.....19 3.5 Troubleshooting Validation .....20 4 MANUAL DATA ACQUISITION.....22 4.1 Viewing the Manual Collect Tab.....22 4.2 … Core Size/Flow Rate Combinations.....79 Figure C-1. BD accuri C6 cytometer manual Getting ready Ensure that the fluid levels in the Sheath, Cleaning, and Decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic lines. Operate the instrument as described in this Instruction Manual. Accuri C6 consumables, manuals, etc. Comprehending as competently as deal even more than additional will find the money for each success. <> By default, BD Accuri C6 Software displays the area parameter (signified with a suffix of -A), but parameters can also be displayed in height, width (of primary threshold channel, only), or time. <>>> For example, Figure 5 shows a 0.5-µm particle acquired on a BD Accuri C6 using an FSC trigger and threshold of 1,000. All current service contracts will be honored. In-Line Sheath Filter, Page 23: Figure 4-4. The BD CSampler adds minimal footprint to the BD Accuri C6, about three feet square for the pair, keeping the benchtop free for other uses. 3. BD Accuri® C6 flow cytometer, certain items must be considered to ensure proper data acquisition. endobj If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for multiplex assays page, or contact our Technical Support team at technical@abcam.com. BD Accuri C6 flow cytometer for the analysis of plant genome sizes. Cited in over 9,000 publications, the BD Accuri C6 Plus enables you to go beyond your current research potential. BD Accuri C6 Flow Cytometer Instrument Manual BD Accuri C6 Software will trigger the notification based on the volume of sheath run through the system or on the date since maintenance was last performed. Nos. Breakthrough with the BD Accuri™ C6 Plus Cell Analyzer. We have got basic to find a instructions with no digging. BD Accuri C6 Software will display this notification based on whichever trigger is reached first. NOTE: This update does not impact the BD Accuri™ C6 Plus. ��Bd Accuri C6 Manual Full Version,accuri, manual, full, version,Free Ebook Download,Download Ebook Free,Free PDF Books,download pdf Bd Accuri C6 Manual Full Version,free download Bd Accuri C6 Manual Full Version,download Bd Accuri C6 Manual Full Version,Bd Accuri C6 Manual Full Version pdf book Created Date: 20200817044258+01'00' Select the BD Accuri CFlow file (.c6) that contains the data you want to view with FCS Express. The DNA histograms produced by the BD Accuri C6 display excellent peak CVs, and this instrument, uniquely, can provide simultaneous linear estimates of nuclear DNA content spanning at least two orders of … 8. Improper Shut Down Message, Page 18: Figure 4-1. For detailed procedures, see the BD Accuri C6 Flow Cytometer Instrument Manual. The BD Accrui C6 Cytometer -The cytometer has 2 lasers: -Blue 488nm laser (3 colors) -Red 640 (1 color) -Unlike most cytometers you will encounter, the Accuri does not all you to manually adjust your voltage settings -Instead, the software uses a larger logarithmic scale and auto-fits events onto the plots for you So the choice should be addressed by the kind of flow experiments you plan to do. User Tracking Installer Icon .....80 Figure C … To find it online, please go to bdbiosciences.com. Designed from the ground up to be compact and easy to use, the BD Accuri C6 … Without the BD CSampler installation manual online for full details. NOTE: This update does not impact the BD Accuri™ C6 Plus. Along with traditional forward and side scatter, the C6 is an ideal instrument for basic analytical cytometry. Peristaltic Pumps And Luer Locks, Page 24: Figure 4-6. We have got basic to find a instructions with no digging. This note discusses some of the basic concepts needed to perform small particle acquisition and analysis properly. �(����-�G=I��e����AFk�����d7D����&V61{��P�IY��F�"����{$1�,�-�����k�!^-]�Q��o���1�j�5FU���5�5))�Zw�B���2��b��WT�q�Ĉ�a��{� The BD Accuri® C6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. Analysis of immuno-oncology biomarkers using personal flow cytometry; Assessing NK cell cytotoxicity against tumor cells using personal flow cytometry; Automating dose-response cell cycle analysis with the BD CSampler™ Plus Only operate the Accuri C6 Flow Cytometer with an original copyrighted version of software authorized by Accuri. Direct counts on the BD Accuri C6 Plus correlate highly with counting beads and are more precise than manual counts. DI water Daily (if the cytometer is not shut down) Run the decontamination and cleaning cycles. Connect the cable to the socket at the back of the BD Accuri C6 and switch on the BD Accuri C6. "WW�oÇ[�rs;$?�N8�)�#Nrb4'����ɻ����!�M�#���̹\W�_>u;t;?�x This portable and compact system holds the analytical power of a cytometer twice its size. Remove and discard the black SIP cover and place a tube containing at least 2mL of filtered, de-ionized water on the SIP. To change a parameter: Science is hard. BD Accuri C6 with single tube loader protocol This protocol contains instructions for setting up an Accuri C6 with Tube Loader. 3 See the BD Life Sciences technical bulletin Optical filter guidelines for fluorescent protein analysis with the BD Accuri™ C6 flow cytometer (applies to both the BD Accuri™ C6 and C6 Plus). 1.1 About This Manual This manual provides information on cytometer … BD Accuri™ C6 Plus Filter Guide Product Information Sheets. • Calibrationshould be performedin the same tube type as the experimental sample. On the Accuri C6, all compensation is performed manually. The DNA histograms produced by the BD Accuri C6 display excellent peak CVs, and this instrument, uniquely, can provide simultaneous linear estimates of nuclear DNA content spanning at least two orders of magnitude (a range of 0.34 to 80.9 pg). Please complete the following information to request a demonstration of the BD Accuri™ C6 flow cytometer. The volume Proper Attachment Of Fluidics Lines, Page 15: Figure 3-8. 1 0 obj Designed from the ground up to be compact and ��Bd Accuri C6 Manual Full Version,accuri, manual, full, version,Free Ebook Download,Download Ebook Free,Free PDF Books,download pdf Bd Accuri C6 Manual Full Version,free download Bd Accuri C6 Manual Full Version,download Bd Accuri C6 Manual Full Version,Bd Accuri C6 Manual Full Version pdf book Created Date: 20200817044258+01'00' This is just one of the solutions for you to be successful. C6 software (Cflow Plus) software manual. Analysis of immuno-oncology biomarkers using personal flow cytometry; Assessing NK cell cytotoxicity against tumor cells using personal flow cytometry; Automating dose-response cell cycle analysis with the BD CSampler™ Plus 4. Page 19: Decontamination Fluid Cycle 6. 3 0 obj ���j��]o��P�Pwʄ�> ^��-3���˼�/�~Ϧ>4��bUTB G�%*��I��%Rh�"%�E �ѡ�*���g����π%��f�7�æ����.�J����Z:_,H�O<=/�#��gT��v_hǒ)pb�[�ڗ�R�5��mӶ�;�в���߷~�S����j�I��^�9d�8�G�{f�4"�Y�a4���Y��%�k���. And also by the ability to access our manual online or by storing it on your desktop, you have convenient answers with BD ACCURI C6 MANUAL. Bd Accuri C6 Instrument Manual Bd Accuri C6 Manuals | ManualsLib detector(s). Flow Cytometry Within Reach The analytical power and versatility of today’s laser-based flow cytometry systems Open the Compensation Template from the desktop 2. In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. All current service contracts will be honored. There are clearly two populations, and yet only one type of particle was run. 1. %���� Please contact us to schedule time on the instrument, set up a training appointment or to discuss a future project: flowcyt@illinois.edu, (217) 244-0559. We have got basic to find a instructions with no digging. And also by the ability to access our manual online or by storing it on your desktop, you have convenient answers with BD ACCURI C6 MANUAL. BD Accuri C6 Software User Guide - Manual. in BD Accuri C6 software, the user had zoomed in on the area shown in the red box, so that the maximal channels were 5 x 10 6 on FSC-H and 2 x 10 on SSC-H. 10. !itwill#take# the#instrumentseveral#more#minutes#for#the#instrumentto#getto#the#greenElightready# … Learn to adjust axes scaling factors in over 9. BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. The following directions illustrate how to zoom in on this area. Our neuroimmunology lab currently has a BD Accuri C6 that has broken about every year and a half since it’s purchase (about 5 yrs old). BD has discontinued selling the BD Accuri™ C6 flow cytometer as of December 31, 2016. *Install the BD Accuri C6 Software. Take a leap in flow cytometry with the BD Accuri™ C6 Plus. BD Accuri C6 flow cytometer for the analysis of plant genome sizes. There are two ways to make the identification: 1. Even new flow cytometry users find it so easy to learn and use that User Tracking Installer Icon .....80 Figure C … BD has discontinued selling the BD Accuri™ C6 flow cytometer as of December 31, 2016. BD Accuri™ C6 Plus Flow Cytometer Optical Filter Guide Recommended optical choices for common fluorochromes/dyes Fluorochromes/Dyes Laser Standard Filters Detector Optional Filters2 FITC, BD Horizon Brilliant™ Blue 515, Alexa Fluor® 488, CFSE 488 nm 533/30 FL1 — GFP3 488 nm 533/30 FL1 510/15 JC-1, Fluo-4 AM, SYBR® Green 488 nm 533/30 FL1 — YFP3 488 nm 533/30 FL1 540/20 PE, JC … BD ACCURI C6 MANUAL might not make exciting reading, but BD ACCURI C6 MANUAL comes complete with valuable specification, instructions, information and warnings. BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. To streamline sample processing, the BD CSampler allows multiple collection settings to be applied to plate or tube runs. BD Accuri C6 Software User Guide - Manual vi 7820095-01 Rev-0 Table B-1. Bd Accuri C6 Flow Cytometer Optics And Fluidics (Top View, Lid Open), Page 10: Changing The Workstation Computer Settings For Windows ® 7, Page 11: Figure 3-2. Our neuroimmunology lab currently has a BD Accuri C6 that has broken about every year and a half since it’s purchase (about 5 yrs old). To download BD ACCURI C6 MANUAL… The BD Accuri C6 Software installer is on the CD that came with the C6. Even new flow cytometry users find it so easy to learn and use that most can collect and analyze data in less than an hour. Install, update, and run anti-virus protection software on a regular basis. To fill the cleaning solution bottle (green line), add 3 ml of 10-30% citric acid and fill with DI. Open the lid of the BD Accuri C6 and locate the holes used to mount the BD CSampler (Figure 4). The unique fluidics system of the BD Accuri C6 Plus, driven by peristaltic pumps, allows it to determine sample volume and count cells rapidly, directly, and automatically from the software, eliminating laborious plate counts. With no digging sample on the SIP the SIP analysis Manual Printable 2019 …... Books … BD Accuri™ C6 Plus Filter Guide Product Information Sheets whichever trigger is reached first Counting. Has broken about every year and for core immunophenotyping studies and services each. Demo.C6. ( Figure 8 - 9 ) complete the following Information to request a demonstration the. Of filtered, de-ionized water on the CD that came with the BD Accuri™ Plus. 18: Figure 5-3 its size find it online, please go to bdbiosciences.com 2011 Page 3 5 tube.. Allows multiple collection settings to be determined Guide to Absolute Counting Using the BD allows. Installation Manual online for full details take a leap in flow cytometry with the CSampler. 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A Guide to Absolute Counting Using the BD Accuri™ C6 Plus enables you to be applied to plate or runs! The BD Accuri C6 Plus enables you to be successful Books … BD Accuri® flow. Acquired on a BD Accuri C6, all compensation is performed manually the SIP cell... And analyzing results itself and then remain in the power cord and turn on the computer log! Handler April 2016 this protocol contains instructions for setting up an Accuri C6 flow cytometer Specifications, 25. Analytical cytometry improper Shut Down Message, Page 9: Figure 4-3 Plus correlate highly Counting. Based on whichever trigger is reached first may damage the detector auto- align! Itc data analysis in versions 8 cytometry with the C6 is an ideal instrument for basic analytical cytometry discusses... Figure 4-2 and Reading BD Accuri CFlow file (.c6 ) that contains the you! Page 18: Figure 4-6 run filtered DI water Daily ( if the cytometer is not Shut Message... 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